Comparative analysis of boar seminal plasma proteome from different freezability ejaculates and identification of Fibronectin 1 as sperm freezability marker
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چکیده
منابع مشابه
Sperm transcriptome profiling for assessment of boar semen freezability
In this project transcriptome analysis on highthroughput RNA-sequencing (RNA-Seq) data of boar spermatozoa will be utilized to identify novel genetic markers associated with semen freezability. Three boars each with poor and good semen freezability will be subjected to transcriptome studies on sperm RNA-seq data, and in combination with bioinformatics screening, will select candidate genes asso...
متن کاملFertility after post-cervical artificial insemination with cryopreserved sperm from boar ejaculates of good and poor freezability.
This study compared the field fertility outcomes in frozen-thawed (FT) sperm from boar ejaculates with different freezability (good, GFE/poor, PFE) while testing the reliability of the post-cervical artificial insemination (post-CAI) in FT sperm. The assay was conducted over eight months with 86 weaned sows being inseminated by post-CAI. Every ejaculate in a total of 26 from 15 Piétrain boars w...
متن کاملMeasurement of activity and concentration of paraoxonase 1 (PON-1) in seminal plasma and identification of PON-2 in the sperm of boar ejaculates.
This study revealed and characterised the presence of the antioxidant enzymes paraoxonase (PON) type 1 (PON-1, extracellular) and type 2 (PON-2, intracellular) in boar semen. To evaluate PON-1, an entire ejaculate from each of ten boars was collected and the seminal plasma was harvested after double centrifugation (1,500g for 10 min). Seminal plasma was analysed for concentration as well as enz...
متن کاملDetrimental Effects of Non-Functional Spermatozoa on the Freezability of Functional Spermatozoa from Boar Ejaculate
In the present study, the impact of non-functional spermatozoa on the cryopreservation success of functional boar spermatozoa was evaluated. Fifteen sperm-rich ejaculate fractions collected from five fertile boars were frozen with different proportions of induced non-functional sperm (0--native semen sample-, 25, 50 and 75% non-functional spermatozoa). After thawing, the recovery of motile and ...
متن کاملSperm Flagellum Volume Determines Freezability in Red Deer Spermatozoa
The factors affecting the inter-individual differences in sperm freezability is a major line of research in spermatology. Poor sperm freezability is mainly characterised by a low sperm velocity, which in turn is associated with low fertility rates in most animal species. Studies concerning the implications of sperm morphometry on freezability are quite limited, and most of them are based on spe...
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ژورنال
عنوان ژورنال: Andrology
سال: 2015
ISSN: 2047-2919
DOI: 10.1111/andr.12009